Effect of Different Solvents on Extraction of Phenolic Antioxidants by Reflux Method from Wild Pomegranate Flavedo, their Antioxidant and Antibacterial Properties

In this study wild pomegranate flavedo powder was used for the extraction of phenolics through various solvents by reflux method. A significant difference in all parameters observed among different extracts with varying and time after oven drying. The highest total phenolics, flavonoids, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, ferric reducing antioxidant power metal chelating activity were recorded when combination ethanol distilled water ratio 50:50 (1 h time) Cluster analysis based on Ward’s method showed that treatments divided nine major groups three clusters application cluster analysis. two best selected further compared drying lyophilizer tested against Staphylococcus aureus Escherichia coli antibacterial activity. conclusion, lyophilized extract contained higher bioactive compounds than dried extract, which have effects could be useful natural antioxidants. concluded fruit is rich source flavonoids beneficial antioxidants health many promoting properties makes suitable future processing into functional/nutraceutical foods.